Total Antioxidant Status (TAS) Kiti

A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation

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Açıklama

EREL’S METHOD

A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation

Clin Biochem. 2004 Apr;37(4):277-85

Dünyada ilk ve tek ayraçları, standartları ve iki seviye kontrolleri tamamen likit kit

  • Toplam antioksidan kapasite direk ölçüm kiti
  • Kullanıma hazır
  • Kolorimetrik
  • Herhangi bir biyokimya otoanalizöründe tam otomatik olarak veya spektrofotometre ve eliza okuyucuda çalışır.
  • Kısa sürede ölçüm
  • En uzun ömürlü ve en dayanıklı ayraç ve standartlar
  • Serum, heparinli plazma, plevra sıvısı, beyin omurilik sıvısı, amnios sıvısı, semen plazması, tükürük ve idrar gibi vücut sıvılarında çalışılabilirler
  • Doku örneklerinde, hücre lizatlarında ve hücre mediumlarında çalışabilirler.
  • Bitki ve gıda ekstraktlarında çalışılabilirler
  • Yağlarda çalışılabilirler. (ayrıca solüsyon istenmelidir)
  • Düşük %CV, yüksek doğrusallık
  • Kolay uygulanabilir, güvenilir ve duyarlıdır.

Kit İçeriği

  1. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Third ed. Oxford: Oxford Science Publications; 2000. p. 617–24.
  2. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176–86.
  3. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004 Apr;37(4):277-85.
  4. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54(5):356 – 61.
  5. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70– 6.
  6. Aycicek A, Iscan A, Erel O, Akcali M, Selek S. Total antioxidant/oxidant status in meningism and meningitis. Pediatr Neurol. 2006 Dec;35(6):382-6.
  7. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004 Feb;37(2):112-9.

Kılavuz

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Bilimsel Yayınlar

Evaluation of oxidant and antioxidant status and relation with prolidase in systemic sclerosis

E Savas, N Aksoy, Y Pehlivan, ZA Sayiner… – Wiener klinische …, 2014 – Springer
… The TOS of serum was determined using a novel-automated measurement method, also
developed by Erel [16], the Rel Assay (Rel Assay Diagnostics kit, Mega Tip, Gaziantep, Turkey) …
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Ö PirgonH Bilgin, F Çekmez, H Kurku… – Journal of clinical …, 2013 – ncbi.nlm.nih.gov
… Plasma total antioxidant status (TAS) and total oxidant status (TOS) level measurements (REL
Assay Diagnostics) were done in all participants … TAS Determination. TAS was measured
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H Toker, A Akpınar, H Aydın… – Journal of periodontal …, 2012 – Wiley Online Library
… TOS and TAS levels were measured using commercially available kits (Rel Assay, Mega Tıp,
Gaziantep, Turkey) by Erel’s colorimetric method (21,22) at an … Table 3. Interleukin-1beta (IL-1β)
level, total oxidant status (TOS) and total antioxidant status (TAS) in gingival …  Cited by 40 Related articles All 7 versions

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G Özkan, S Ulusoy, M Alkanat, A Orem, B Akcan… – Renal failure, 2012 – Taylor & Francis
… Tissue TAS, TOS, and OSI levels were assayed as previously described,11 using commercial
assay kits (Rel Assay Diagnostics, Gaziantep … Notes: CsA, cyclosporine A; GSPE, grape seed
proanthocyanidin extract; TOS, total oxidant system; TAS, total antioxidant system; OSI …  Cited by 24 Related articles All 3 versions

Evaluation of total oxidative stress and total antioxidant status in cows with natural bovine herpesvirus-1 infection

R Durgut, VS Ataseven, A Sağkan-Öztürk… – Journal of animal …, 2013 – academic.oup.com
… E and C increased antioxidant capacity. Pagnini et al. (2004) indicated that antioxidants prevented
oxidative stress; however, they failed to stop virus growth. The objective of this study is to
demonstrate the relationship among total antioxidative status (TAS), total oxidant status …  Cited by 13 Related articles All 8 versions

The protection of selenium on adriamycin-induced mitochondrial damage in rat

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… Mitochondrial ATP content from each probe was assessed in duplicate. Biochemical Analysis.
Measurement of Total Antioxidant Status. Total antioxidant status (TAS) of the cytosol and
of the kidney cell mitochondria were measured using Rel Assay Kit …  Cited by 26 Related articles All 10 versions

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S Ozturk, O Ozcan, AB Gurpinar, Y Can… – … journal of clinical and …, 2014 – ncbi.nlm.nih.gov
… from the cells by centrifugation at 3000 rpm for 15 min and were stored at -80°C and used for
the analysis of the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS). Analysis of
blood samples. Serum TAS and TOS were determined with kits (Rel Assay Diagnostics kit …  Cited by 23 Related articles All 10 versions

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NC Yildirim, S Turkoglu, N Yildirim… – Digest Journal of …, 2012 – chalcogen.ro
… 2.7 Total Antioxidant Status (TAS) Total antioxidant status was determined by using Rel assay
diagnostics tas assay kit (Lot.RL024) by Multiscan FC (Thermo). Antioxidants in the sample reduce
dark blue-green colored ABTS radical to colorless reduced ABTS form …  Cited by 19 Related articles All 4 versions [PDF] hindawi.com

The association between diabetic retinopathy and levels of ischemia-modified albumin, total thiol, total antioxidant capacity, and total oxidative stress in serum and …

K Kirboga, AV Ozec, M Kosker, A Dursun… – Journal of …, 2014 – hindawi.com
… equiv./L). 2.3.4. Total Antioxidant Status Assay. TAS was determined using commercial
Rel Assay Diagnostic kits (Gaziantep, Turkey) with an autoanalyzer LX20-Pro from
Beckman-Coulter (Woerden, The Netherlands). The method …  Cited by 9 Related articles All 12 versions [PDF] researchgate.net

Effect of Low‐Dose Doxycycline on Serum Oxidative Status, Gingival Antioxidant Levels, and Alveolar Bone Loss in Experimental Periodontitis in Rats

A Yağan, S Kesim, N Liman – Journal of periodontology, 2014 – Wiley Online Library
… Fully Automated 3rd Generation Total Antioxidant Status (TAS) Assay Kit, Product Code RL0017,
Rel Assay Diagnostics, Mega Tıp, Gaziantep, Turkey. ## Fully Automated Total Oxidant Status
(TOS) Assay Kit, Product Code RL0024, Rel Assay Diagnostics, Mega Tıp …  Cited by 36 Related articles All 8 versions

İçerik

Reagent 1 – Buffer 1 x 30 ml
Reagent 2 – ABTS Radical Cation 1 x 8 ml
Standard 1 x 4 ml
Quality Control – Level 1 1 x 4 ml
Quality Control – Level 2 1 x 4 ml

Referanslar

  1. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Third ed. Oxford: Oxford Science Publications; 2000. p. 617–24.
  2. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176–86.
  3. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004 Apr;37(4):277-85.
  4. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54(5):356 – 61.
  5. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70– 6.
  6. Aycicek A, Iscan A, Erel O, Akcali M, Selek S. Total antioxidant/oxidant status in meningism and meningitis. Pediatr Neurol. 2006 Dec;35(6):382-6.
  7. 7.Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004 Feb;37(2):112-9.