Testosterone ELISA Kit (Universal)

Ürün arzı gerçekleştirdiğimiz gibi; numunelerinizi -78°C ile aldırıp; laboratuvarımızda numunelerinizi çalışma seçeneğimiz de bulunmaktadır. Çalışma esnasında bulunmak isteyen bilim insanlarını misafir etmekten büyük keyif duyacağımızı belirtmek isteriz.

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Açıklama

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated.

Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T in tested samples can be calculated by comparing the OD of the samples to the standard curve.